Chemical compositions and larvicidal effectiveness on nerium oleander flower essential oil on hyphantria cunea larvae
1 Artvin Coruh University, Faculty of Forestry, Department of Forestry Engineering, Artvin, Turkey
2 Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi, Georgia
2 Batumi Shota Rustaveli State University, Faculty of Natural Sciences and Health Care, Department of Biology, Batumi, Georgia
Abstract
In this research, the chemical composition of oleander (Nerium oleander L) (Apocynaceae) flower essential oil was analyzed using GC-MS (Gas Chromatography-Mass Spectrometer), and its larvicidal effectiveness on five Fall webworm (Hyphantria cunea Drury) larval stages was investigated. The essential oil was extracted from the flowers of N. oleander by the hydrodistillation method using the Clevenger apparatus. A total of 48 chemical constituents present in oleander essential oil were identified using Gas Chromatography-Mass Spectrometry analysis. Camphore (10.3%), Digitoxigenine (10.21%), Eugenol (8.61%), Thymol (6.15%), and 1.8-Cineole (5.05%) were found as the primary components of oil. It is observed that the applications of N. oleander essential oil at doses of 10, 15, and 20 L/Petri and the controls at 24, 48, 72, and 96 hours induced fatalities at varied rates (40.4100%) on five H. cunea larval stages (L1-L5). The highest toxicity was identified at L1 (0.56 L/larva), and the lowest toxicity was determined at L5 (12.64 L/larva) according to LD50 and LD90 values. The research was conducted in laboratory settings during 2021-22. In conclusion, N. oleander essential oil showed high toxicity against L1 and L2 larval stages of H. cunea.
Keywords
essential oil; fall webworm; larvicidal effect; oleander